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KMID : 1161519990030040399
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Animal Cells and Systems
1999 Volume.3 No. 4 p.399 ~ p.405
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Characterization of the serotyping and the Plasmid profile of E. coli isolated from foods and clinical specimens
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Kwak Hyo-Shun
Lee Chong-Sam
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Abstract
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Characteristics of the food isolates and the clinical specimens isolates of E. coli harboring virulence factor and their correlations were analyzed. The predominant serogroup were O8 and O27 in the food isolates and O6 and O18 in the clinical isolates, respectively, showing the different patterns in serogrouping between them. In the test of antibiotic susceptibility, the food isolates were resistant to cephalothin, streptomycin, tetracycline and minocycline, and the clinical isolates were resistant to ampicillin, carbenicillin, streptomycin, cephalothin, trimethoprim/sulfamethoxazole, tetracycline and minocycline, respectively. It shows that E.coli isolated from food sources and clinical specimens might be correlated. Plasmid profile in the food and clinical isolates showed wide diversity. Especially, large sized plasmid DNA such as 60 MDa, 90 MDa and 120 MDa were observed. The plasmid DNA (60 MDa) containing a gene encoding hemolysin was found in 43% of the food isolates and 35% of the clinical isolates. To study chromosomal homology, PFGE analysis was performed, showing different restriction patterns by Xbal. This result indicates that there were no genetic correlations between the foods and the clinical isolates.
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KEYWORD
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E. coli, Serotyping, Plasmid profile, Antimicrobial susceptibility
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